Studies on Plant Urease and its Applications

Urease was the first enzyme to be crystallized and the first shown to require nickel ions. Recent interest in urease has centered on its action as a virulence factor, such as its participation in gastroduodenal infections by H. pylori. First chapter of the book deals with the detailed literature review and is divided into three sections: I-urease, II-protein renaturation and III- Biosensors and Immobilization. Chapter 2 is devoted to urease renaturation and is divided into Section I: Investigations of conditions for urease renaturation and Section II: Mechanism of L-Arginine induced renaturation. Chapter 3 deals with Section I: urease immobilization on Cellulose paper strips for urea estimation and Section II: Improved stability of urease upon coupling to alkylamine and arylamine glass and its analytical use. Chapter 4 deals with a single section on urease biosensor. The preparation and characterization of bifunctional needle-type urea microbiosensors are presented. Last chapter (Chapter 5) deals with urease inhibition and is divided into Section I: Urease inhibition by boric and boronic acids Section II: pH inactivation studies.