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This is the fifth volume in the Reviews in Fluorescence series. To date, four volumes have been both published and well received by the scientific community. Several book reviews in the last few years have also favourably remarked on the series.§In this 5th volume we continue the tradition of publishing leading edge and timely articles from authors around the world. We thank the authors for their timely and exciting contributions. We hope you will find this volume as useful as past volumes, which promises to be just as diverse with regard to fluorescence-based content.