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Oryzacystatin-I Expressing Tobacco Plants
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Although several studies have shown increased§tolerance of engineered plants to abiotic stress, §levels of expression and stability of the transgene §are still stumbling blocks to achieve optimal §efficiency. This study has addressed this problem by§studying transgene expression and stability under §stress and their potential benefits to the plants. §In particular, the expression and stability of a §cysteine proteinase inhibitor (OC-I) expressed in §tobacco has been investigated under drought, heat §and combination of both stresses. A specific focus §of the study was on evaluating potential benefits of §the stable expression of OC-I transgene by §measuring growth and physiological performances of §plants under individual and combined stresses. This §also included investigating possible effect of OC-I §overexpression on gene expression in plans. A §further aspect of the study focused on the §effect of OC-I expression in stabilizing plant §proteins. The study finally identified and §characterized two novel papain-like tobacco §cysteine proteinases as possible targets of §expressed OC-I.
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