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Measurement of Cell/Tissue-biomaterial Interface Strength
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A laser spallation technique, which was originally §developed to measure adhesion of thin films, was §adopted to determine the interfacial tensile §strength of cells and mineralized tissue to various §engineered substrates.§Several new experimental equipments, procedures, and §analyzing methods were successfully developed §considering the characteristics of biological §specimens. Also, new machinery of analysis that §enhances the calculation accuracy and efficiency was §developed, and as final calculation methods, both §FEA simulation and analytical method were adopted §and compared with each other.§Interfacial tensile strengths of living cells were §successfully measured, and so were its dependence on §different surface conditions, including polymer and §metal substrates and the presence or absence of §fibronectin treatment and serum.§The feasibility of the living cell isolating and §placing method using the laser spallation technique §was positively demonstrated suggesting the §possibility of the method to enhance existing §individual living cell manipulation techniques for §precise placement.
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