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Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of "Methods in Cell Biology" on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. It covers protocols for cilia and flagella across systems and species. It includes both classic and state-of-the-art methods that are readily adaptable across model systems, and designed to last the test of time. It is relevant to clinicians interested in respiratory disease, male infertility, and other syndromes, who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures.